Properties of the promoter region of the T18d (T13c)Tla gene

Masato Horie, Akihiro Matsuura, King Jen Chang, Junichi Niikawa, Fung Win Shen

研究成果: Article

3 引用 (Scopus)

抄録

The T18d (formerly T13c) gene of BALB/c mice belongs to the category of Tla genes which is expressed by both thymocytes and TL+ T-cell leukemias. To elucidate the regulation of T18d, different restriction fragments of the 5' flanking region between -457 and+ 146 were linked to the chloramphenicol acetyltransferase (CAT) gene and transfected into TL+ and TL- cells. By comparison of transiently expresssed CAT activity among cells transfected with different CAT constructs, the results suggest that determination of TL+ vs TL phenotypes is located within the region -105 to - 33, and that an element essential to T18 d expression resides within the region - 33 to + 54. Putative DNA-binding factors characterizing particular cell types and displaying selective affinity for particular TI 8d restriction fragments were identified by electrophoretic mobility shift assays with nuclear extracts (NEs). Two factors (or complexes) which bound specifically to the T18d fragment-105 to - 33 were expressed preferentially in TL+ cells and thus may be involved in determining the tissue-selective expression of TI8d. The close proximity of negative and positive cis-acting elements within the promoter region is consistent with regulation of T18d gene expression by a variety of trans-acting factors whose production is attuned to development and differentiation. The data provided may serve as a guide to study the regulation of other categories of Tla genes that are normally silent in thymocytes but may become expressed by leukemia cells.

元の言語English
ページ(範囲)171-177
ページ数7
ジャーナルImmunogenetics
33
発行部数3
DOI
出版物ステータスPublished - 01-05-1991
外部発表Yes

Fingerprint

Genetic Promoter Regions
Chloramphenicol O-Acetyltransferase
Genes
Thymocytes
T-Cell Leukemia
Trans-Activators
5' Flanking Region
Gene Expression Regulation
Electrophoretic Mobility Shift Assay
Leukemia
Phenotype
DNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

これを引用

Horie, Masato ; Matsuura, Akihiro ; Chang, King Jen ; Niikawa, Junichi ; Shen, Fung Win. / Properties of the promoter region of the T18d (T13c)Tla gene. :: Immunogenetics. 1991 ; 巻 33, 番号 3. pp. 171-177.
@article{08150c0222b947a4b0b91b89d2c6a701,
title = "Properties of the promoter region of the T18d (T13c)Tla gene",
abstract = "The T18d (formerly T13c) gene of BALB/c mice belongs to the category of Tla genes which is expressed by both thymocytes and TL+ T-cell leukemias. To elucidate the regulation of T18d, different restriction fragments of the 5' flanking region between -457 and+ 146 were linked to the chloramphenicol acetyltransferase (CAT) gene and transfected into TL+ and TL- cells. By comparison of transiently expresssed CAT activity among cells transfected with different CAT constructs, the results suggest that determination of TL+ vs TL phenotypes is located within the region -105 to - 33, and that an element essential to T18 d expression resides within the region - 33 to + 54. Putative DNA-binding factors characterizing particular cell types and displaying selective affinity for particular TI 8d restriction fragments were identified by electrophoretic mobility shift assays with nuclear extracts (NEs). Two factors (or complexes) which bound specifically to the T18d fragment-105 to - 33 were expressed preferentially in TL+ cells and thus may be involved in determining the tissue-selective expression of TI8d. The close proximity of negative and positive cis-acting elements within the promoter region is consistent with regulation of T18d gene expression by a variety of trans-acting factors whose production is attuned to development and differentiation. The data provided may serve as a guide to study the regulation of other categories of Tla genes that are normally silent in thymocytes but may become expressed by leukemia cells.",
author = "Masato Horie and Akihiro Matsuura and Chang, {King Jen} and Junichi Niikawa and Shen, {Fung Win}",
year = "1991",
month = "5",
day = "1",
doi = "10.1007/BF01719236",
language = "English",
volume = "33",
pages = "171--177",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "3",

}

Horie, M, Matsuura, A, Chang, KJ, Niikawa, J & Shen, FW 1991, 'Properties of the promoter region of the T18d (T13c)Tla gene', Immunogenetics, 巻. 33, 番号 3, pp. 171-177. https://doi.org/10.1007/BF01719236

Properties of the promoter region of the T18d (T13c)Tla gene. / Horie, Masato; Matsuura, Akihiro; Chang, King Jen; Niikawa, Junichi; Shen, Fung Win.

:: Immunogenetics, 巻 33, 番号 3, 01.05.1991, p. 171-177.

研究成果: Article

TY - JOUR

T1 - Properties of the promoter region of the T18d (T13c)Tla gene

AU - Horie, Masato

AU - Matsuura, Akihiro

AU - Chang, King Jen

AU - Niikawa, Junichi

AU - Shen, Fung Win

PY - 1991/5/1

Y1 - 1991/5/1

N2 - The T18d (formerly T13c) gene of BALB/c mice belongs to the category of Tla genes which is expressed by both thymocytes and TL+ T-cell leukemias. To elucidate the regulation of T18d, different restriction fragments of the 5' flanking region between -457 and+ 146 were linked to the chloramphenicol acetyltransferase (CAT) gene and transfected into TL+ and TL- cells. By comparison of transiently expresssed CAT activity among cells transfected with different CAT constructs, the results suggest that determination of TL+ vs TL phenotypes is located within the region -105 to - 33, and that an element essential to T18 d expression resides within the region - 33 to + 54. Putative DNA-binding factors characterizing particular cell types and displaying selective affinity for particular TI 8d restriction fragments were identified by electrophoretic mobility shift assays with nuclear extracts (NEs). Two factors (or complexes) which bound specifically to the T18d fragment-105 to - 33 were expressed preferentially in TL+ cells and thus may be involved in determining the tissue-selective expression of TI8d. The close proximity of negative and positive cis-acting elements within the promoter region is consistent with regulation of T18d gene expression by a variety of trans-acting factors whose production is attuned to development and differentiation. The data provided may serve as a guide to study the regulation of other categories of Tla genes that are normally silent in thymocytes but may become expressed by leukemia cells.

AB - The T18d (formerly T13c) gene of BALB/c mice belongs to the category of Tla genes which is expressed by both thymocytes and TL+ T-cell leukemias. To elucidate the regulation of T18d, different restriction fragments of the 5' flanking region between -457 and+ 146 were linked to the chloramphenicol acetyltransferase (CAT) gene and transfected into TL+ and TL- cells. By comparison of transiently expresssed CAT activity among cells transfected with different CAT constructs, the results suggest that determination of TL+ vs TL phenotypes is located within the region -105 to - 33, and that an element essential to T18 d expression resides within the region - 33 to + 54. Putative DNA-binding factors characterizing particular cell types and displaying selective affinity for particular TI 8d restriction fragments were identified by electrophoretic mobility shift assays with nuclear extracts (NEs). Two factors (or complexes) which bound specifically to the T18d fragment-105 to - 33 were expressed preferentially in TL+ cells and thus may be involved in determining the tissue-selective expression of TI8d. The close proximity of negative and positive cis-acting elements within the promoter region is consistent with regulation of T18d gene expression by a variety of trans-acting factors whose production is attuned to development and differentiation. The data provided may serve as a guide to study the regulation of other categories of Tla genes that are normally silent in thymocytes but may become expressed by leukemia cells.

UR - http://www.scopus.com/inward/record.url?scp=0025847638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025847638&partnerID=8YFLogxK

U2 - 10.1007/BF01719236

DO - 10.1007/BF01719236

M3 - Article

C2 - 2010219

AN - SCOPUS:0025847638

VL - 33

SP - 171

EP - 177

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 3

ER -