Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

Takao Tsuji; Takeshi Shimizu; Keiko Sasaki; Yoshiyasu Shimizu; Kentaro Tsukamoto; Hideyuki Arimitsu; Sadayuki Ochi; Satoshi Sugiyama; Koki Taniguchi; Paola Neri; Hiroshi Mori

doi:10.1016/j.vaccine.2007.11.038

Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

Takao Tsuji
, Takeshi Shimizu
, Keiko Sasaki
, Yoshiyasu Shimizu
, Kentaro Tsukamoto
, Hideyuki Arimitsu
, Sadayuki Ochi
, Satoshi Sugiyama
, Koki Taniguchi
, Paola Neri
, Hiroshi Mori

医学部・微生物学

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

12 被引用数 (Scopus)

抄録

Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

本文言語	英語
ページ（範囲）	469-476
ページ数	8
ジャーナル	Vaccine
巻	26
号	4
DOI	https://doi.org/10.1016/j.vaccine.2007.11.038
出版ステータス	出版済み - 24-01-2008

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

SDG 3 すべての人に健康と福祉を

All Science Journal Classification (ASJC) codes

分子医療
免疫学および微生物学一般
獣医学一般
公衆衛生学、環境および労働衛生
感染症

文献へのアクセス

10.1016/j.vaccine.2007.11.038

他のファイルとリンク

引用スタイル

@article{82a160faefc84d888d2659e31a39fe76,

title = "Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin",

abstract = "Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.",

author = "Takao Tsuji and Takeshi Shimizu and Keiko Sasaki and Yoshiyasu Shimizu and Kentaro Tsukamoto and Hideyuki Arimitsu and Sadayuki Ochi and Satoshi Sugiyama and Koki Taniguchi and Paola Neri and Hiroshi Mori",

note = "Funding Information: This work was supported by a grants-in-aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology and Japan Society for the Promotion of Science (JSPS) and for the Open Research Center's Project of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2008",

month = jan,

day = "24",

doi = "10.1016/j.vaccine.2007.11.038",

language = "English",

volume = "26",

pages = "469--476",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier Ltd",

number = "4",

}

TY - JOUR

T1 - Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

AU - Tsuji, Takao

AU - Shimizu, Takeshi

AU - Sasaki, Keiko

AU - Shimizu, Yoshiyasu

AU - Tsukamoto, Kentaro

AU - Arimitsu, Hideyuki

AU - Ochi, Sadayuki

AU - Sugiyama, Satoshi

AU - Taniguchi, Koki

AU - Neri, Paola

AU - Mori, Hiroshi

N1 - Funding Information: This work was supported by a grants-in-aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology and Japan Society for the Promotion of Science (JSPS) and for the Open Research Center's Project of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2008/1/24

Y1 - 2008/1/24

N2 - Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

AB - Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

UR - https://www.scopus.com/pages/publications/37849003390

UR - https://www.scopus.com/pages/publications/37849003390#tab=citedBy

U2 - 10.1016/j.vaccine.2007.11.038

DO - 10.1016/j.vaccine.2007.11.038

M3 - Article

C2 - 18093704

AN - SCOPUS:37849003390

SN - 0264-410X

VL - 26

SP - 469

EP - 476

JO - Vaccine

JF - Vaccine

IS - 4

ER -

Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

抄録

UN SDG

All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル