TY - JOUR
T1 - Protein kinase C and calcium ion in mitogenic response of macrophage-depleted human peripheral lymphocytes
AU - Kaibuchi, K.
AU - Takai, Y.
AU - Nishizuka, Y.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1985
Y1 - 1985
N2 - For mitogenic response of macrophage-depleted human peripheral lymphocytes, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or 1-oleoyl-2-acetylglycerol (OAG) and Ca2+ ionophore are both needed in addition to a small quantity of plant lectin, phytohemagglutinin (PHA). PHA alone is not sufficient to produce the cellular response. The addition of TPA or OAG to these cells induces the activation of protein kinase C as assayed by the phosphorylation of its endogenous substrates. Apparently, TPA or OAG and A23187 together substitute for macrophages and act synergistically to potentiate the DNA synthesis of this lymphocyte preparation. The results suggest that protein kinase C activation and Ca2+ mobilization are essential and that additional receptor occupation by PHA is necessary for producing cell proliferation.
AB - For mitogenic response of macrophage-depleted human peripheral lymphocytes, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or 1-oleoyl-2-acetylglycerol (OAG) and Ca2+ ionophore are both needed in addition to a small quantity of plant lectin, phytohemagglutinin (PHA). PHA alone is not sufficient to produce the cellular response. The addition of TPA or OAG to these cells induces the activation of protein kinase C as assayed by the phosphorylation of its endogenous substrates. Apparently, TPA or OAG and A23187 together substitute for macrophages and act synergistically to potentiate the DNA synthesis of this lymphocyte preparation. The results suggest that protein kinase C activation and Ca2+ mobilization are essential and that additional receptor occupation by PHA is necessary for producing cell proliferation.
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M3 - Article
C2 - 3155734
AN - SCOPUS:0021913289
SN - 0021-9258
VL - 260
SP - 1366
EP - 1369
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 3
ER -