Protein kinase C and calcium ion in mitogenic response of macrophage-depleted human peripheral lymphocytes

K. Kaibuchi, Y. Takai, Y. Nishizuka

研究成果: Article査読

140 被引用数 (Scopus)

抄録

For mitogenic response of macrophage-depleted human peripheral lymphocytes, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or 1-oleoyl-2-acetylglycerol (OAG) and Ca2+ ionophore are both needed in addition to a small quantity of plant lectin, phytohemagglutinin (PHA). PHA alone is not sufficient to produce the cellular response. The addition of TPA or OAG to these cells induces the activation of protein kinase C as assayed by the phosphorylation of its endogenous substrates. Apparently, TPA or OAG and A23187 together substitute for macrophages and act synergistically to potentiate the DNA synthesis of this lymphocyte preparation. The results suggest that protein kinase C activation and Ca2+ mobilization are essential and that additional receptor occupation by PHA is necessary for producing cell proliferation.

本文言語English
ページ(範囲)1366-1369
ページ数4
ジャーナルJournal of Biological Chemistry
260
3
出版ステータスPublished - 1985
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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