Protein synthesis in isolated enterocytes from septic or endotoxaemic rats

Regulation by glutamine

Takashi Higashiguchi, Y. Noguchi, T. Meyer, J. E. Fischer, P. O. Hasselgren

研究成果: Article

31 引用 (Scopus)

抄録

We studied the effect of sepsis and the regulation by glutamine of protein synthesis in enterocytes isolated from the small intestine of rats. Sepsis was induced by caecal ligation and puncture; control rats were sham operated. Enterocytes were isolated from the jejunum and incubated in a medium containing [3H]phenylalanine. Sixteen hours after caecal ligation and puncture, protein synthesis, measured as incorporation of radioactivity into protein, was increased by 65%, 89% and 137% respectively in enterocytes from the tips and mid-portions of the villi and from the crypts. Addition of glutamine to incubated enterocytes stimulated protein synthesis in a dose-dependent manner, and this effect was most pronounced in crypt cells from septic rats. The effect of glutamine on protein synthesis was duplicated by equimolar concentrations of acetoacetate or 3-hydroxybutyrate, both of which may serve as fuel for enterocytes, and was blocked by the glutaminase inhibitor 6-diazo-5-oxo-L-norleucine. The results suggest that sepsis stimulates protein synthesis in enterocytes and that glutamine regulates protein synthesis in the same cells, probably by energy provision.

元の言語English
ページ(範囲)311-319
ページ数9
ジャーナルClinical Science
89
発行部数3
DOI
出版物ステータスPublished - 01-01-1995
外部発表Yes

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Enterocytes
Glutamine
Proteins
Sepsis
Punctures
Ligation
Diazooxonorleucine
Glutaminase
3-Hydroxybutyric Acid
Jejunum
Phenylalanine
Radioactivity
Small Intestine

All Science Journal Classification (ASJC) codes

  • Medicine(all)

これを引用

Higashiguchi, Takashi ; Noguchi, Y. ; Meyer, T. ; Fischer, J. E. ; Hasselgren, P. O. / Protein synthesis in isolated enterocytes from septic or endotoxaemic rats : Regulation by glutamine. :: Clinical Science. 1995 ; 巻 89, 番号 3. pp. 311-319.
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Protein synthesis in isolated enterocytes from septic or endotoxaemic rats : Regulation by glutamine. / Higashiguchi, Takashi; Noguchi, Y.; Meyer, T.; Fischer, J. E.; Hasselgren, P. O.

:: Clinical Science, 巻 89, 番号 3, 01.01.1995, p. 311-319.

研究成果: Article

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