TY - JOUR
T1 - Proteomic analysis of human brain identifies α-enolase as a novel autoantigen in Hashimoto's encephalopathy
AU - Ochi, Hirofumi
AU - Horiuchi, Izumi
AU - Araki, Norie
AU - Toda, Tosifusa
AU - Araki, Tomohiro
AU - Sato, Kaori
AU - Murai, Hiroyuki
AU - Osoegawa, Manabu
AU - Yamada, Takeshi
AU - Okamura, Ken
AU - Ogino, Tomoaki
AU - Mizumoto, Kiyohisa
AU - Yamashita, Hirohumi
AU - Saya, Hideyuki
AU - Kira, Jun ichi
N1 - Funding Information:
This work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan (12557060, 12470142 to J.K., and 12671369), a Neuroimmunological Disease Research Committee grant and a Research on Brain Science grant from the Ministry of Health and Welfare of Japan (to J.K.), and a Grant-in-Aid for Scientific Research on Priority Areas(C)–Advanced Brain Science Project–from Ministry of Education, Culture, Sports, Science and Technology, Japan (to N.A.).
PY - 2002/9/25
Y1 - 2002/9/25
N2 - Hashimoto's encephalopathy (HE) is a rare autoimmune disease associated with Hashimoto's thyroiditis (HT). To identify the HE-related autoantigens, we developed a human brain proteome map using two-dimensional electrophoresis and applied it to the immuno-screening of brain proteins that react with autoantibodies in HE patients. After sequential MALDI-TOF-MASS analysis, immuno-positive spots of 48 kDa (pI 7.3-7.8) detected from HE patient sera were identified as a novel autoimmuno-antigen, α-enolase, harboring several modifications. Specific high reactivities against human α-enolase were significant in HE patients with excellent corticosteroid sensitivity, whereas the patients with fair or poor sensitivity to the corticosteroid treatment showed less reactivities than cut-off level. Although a few HT patients showed faint reactions to α-enolase, 95% of HT patients, patients with other neurological disorders, and healthy subjects tested were all negative. These results suggest that the detection of anti-α-enolase antibody is useful for defining HE-related pathology, and this proteomic strategy is a powerful method for identifying autoantigens of various central nervous system diseases with unknown autoimmune etiologies.
AB - Hashimoto's encephalopathy (HE) is a rare autoimmune disease associated with Hashimoto's thyroiditis (HT). To identify the HE-related autoantigens, we developed a human brain proteome map using two-dimensional electrophoresis and applied it to the immuno-screening of brain proteins that react with autoantibodies in HE patients. After sequential MALDI-TOF-MASS analysis, immuno-positive spots of 48 kDa (pI 7.3-7.8) detected from HE patient sera were identified as a novel autoimmuno-antigen, α-enolase, harboring several modifications. Specific high reactivities against human α-enolase were significant in HE patients with excellent corticosteroid sensitivity, whereas the patients with fair or poor sensitivity to the corticosteroid treatment showed less reactivities than cut-off level. Although a few HT patients showed faint reactions to α-enolase, 95% of HT patients, patients with other neurological disorders, and healthy subjects tested were all negative. These results suggest that the detection of anti-α-enolase antibody is useful for defining HE-related pathology, and this proteomic strategy is a powerful method for identifying autoantigens of various central nervous system diseases with unknown autoimmune etiologies.
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U2 - 10.1016/S0014-5793(02)03307-0
DO - 10.1016/S0014-5793(02)03307-0
M3 - Article
C2 - 12297304
AN - SCOPUS:18644372755
SN - 0014-5793
VL - 528
SP - 197
EP - 202
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -
