Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy

Hiromi Nakai-Shimoda, Tatsuhito Himeno, Mikio Motegi, Norio Ozeki, Rieko Inoue, Tomohide Hayami, Emiri Miura-Yura, Yuichiro Yamada, Yoshiaki Morishita, Shin Tsunekawa, Yoshiro Kato, Yusuke Seino, Masaki Kondo, Keiko Naruse, Koichi Kato, Hiroki Mizukami, Jiro Nakamura, Hideki Kamiya

研究成果: Article査読

抄録

Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).

本文言語English
論文番号101591
ジャーナルSTAR Protocols
3
3
DOI
出版ステータスPublished - 16-09-2022

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

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