Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

Makito Tanaka; Yoshiyuki Takahashi; Yin Yan Xu; Nao Yoshida; Ayami Yoshimi; Itzel Bustos Villalobos; Asahito Hama; Nobuhiro Nishio; Hirokazu Hidaka; Yue Wang; Hiroshi Yagasaki; Seiji Kojima

doi:10.1016/j.leukres.2007.11.016

Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay

Makito Tanaka, Yoshiyuki Takahashi, Yin Yan Xu, Nao Yoshida, Ayami Yoshimi, Itzel Bustos Villalobos, Asahito Hama, Nobuhiro Nishio, Hirokazu Hidaka, Yue Wang, Hiroshi Yagasaki, Seiji Kojima

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

4 被引用数 (Scopus)

抄録

Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a ³H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

本文言語	英語
ページ（範囲）	1036-1042
ページ数	7
ジャーナル	Leukemia Research
巻	32
号	7
DOI	https://doi.org/10.1016/j.leukres.2007.11.016
出版ステータス	出版済み - 07-2008
外部発表	はい

All Science Journal Classification (ASJC) codes

血液学
腫瘍学
癌研究

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

文献へのアクセス

10.1016/j.leukres.2007.11.016

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引用スタイル

Tanaka, M., Takahashi, Y., Xu, Y. Y., Yoshida, N., Yoshimi, A., Villalobos, I. B., Hama, A., Nishio, N., Hidaka, H., Wang, Y., Yagasaki, H., & Kojima, S. (2008). Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay. Leukemia Research, 32(7), 1036-1042. https://doi.org/10.1016/j.leukres.2007.11.016

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title = "Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay",

abstract = "Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.",

keywords = "Colony-forming unit granulocyte-macrophage (CFU-GM), Granulocyte-macrophage colony-stimulating factor (GM-CSF), H-thymidine assay, Hypersensitivity, Juvenile myelomonocytic leukemia (JMML), PTPN11 mutation, RAS mutation",

author = "Makito Tanaka and Yoshiyuki Takahashi and Xu, {Yin Yan} and Nao Yoshida and Ayami Yoshimi and Villalobos, {Itzel Bustos} and Asahito Hama and Nobuhiro Nishio and Hirokazu Hidaka and Yue Wang and Hiroshi Yagasaki and Seiji Kojima",

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Tanaka, M, Takahashi, Y, Xu, YY, Yoshida, N, Yoshimi, A, Villalobos, IB, Hama, A, Nishio, N, Hidaka, H, Wang, Y, Yagasaki, H & Kojima, S 2008, 'Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay', Leukemia Research, vol. 32, no. 7, pp. 1036-1042. https://doi.org/10.1016/j.leukres.2007.11.016

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AU - Tanaka, Makito

AU - Takahashi, Yoshiyuki

AU - Xu, Yin Yan

AU - Yoshida, Nao

AU - Yoshimi, Ayami

AU - Villalobos, Itzel Bustos

AU - Hama, Asahito

AU - Nishio, Nobuhiro

AU - Hidaka, Hirokazu

AU - Wang, Yue

AU - Yagasaki, Hiroshi

AU - Kojima, Seiji

PY - 2008/7

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N2 - Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

AB - Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

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KW - H-thymidine assay

KW - Hypersensitivity

KW - Juvenile myelomonocytic leukemia (JMML)

KW - PTPN11 mutation

KW - RAS mutation

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