TY - JOUR
T1 - Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170
AU - Fukata, Masaki
AU - Watanabe, Takashi
AU - Noritake, Jun
AU - Nakagawa, Masato
AU - Yamaga, Masaki
AU - Kuroda, Shinya
AU - Matsuura, Yoshiharu
AU - Iwamatsu, Akihiro
AU - Perez, Franck
AU - Kaibuchi, Kozo
N1 - Funding Information:
This research was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and the Japan Society for the Promotion of Science Research for the Future Science.
PY - 2002/6/28
Y1 - 2002/6/28
N2 - Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of IQGAP1, which includes the CLIP-170 binding region, delocalizes GFP-CLIP-170 from the tips of microtubules and alters the microtubule array. Activated Rac1/Cdc42, IQGAP1, and CLIP-170 form a tripartite complex. Furthermore, expression of an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization.
AB - Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of IQGAP1, which includes the CLIP-170 binding region, delocalizes GFP-CLIP-170 from the tips of microtubules and alters the microtubule array. Activated Rac1/Cdc42, IQGAP1, and CLIP-170 form a tripartite complex. Furthermore, expression of an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization.
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U2 - 10.1016/S0092-8674(02)00800-0
DO - 10.1016/S0092-8674(02)00800-0
M3 - Article
C2 - 12110184
AN - SCOPUS:18444369936
SN - 0092-8674
VL - 109
SP - 873
EP - 885
JO - Cell
JF - Cell
IS - 7
ER -