Radial glial cell–neuron interaction directs axon formation at the opposite side of the neuron from the contact site

Chundi Xu, Yasuhiro Funahashi, Takashi Watanabe, Tetsuya Takano, Shinichi Nakamuta, Takashi Namba, Kozo Kaibuchi

研究成果: Article査読

44 被引用数 (Scopus)

抄録

How extracellular cues direct axon–dendrite polarization in mouse developing neurons is not fully understood. Here, we report that the radial glial cell (RGC)–cortical neuron interaction directs axon formation at the opposite side of the neuron from the contact site. N-cadherin accumulates at the contact site between the RGC and cortical neuron. Inhibition of the N-cadherin-mediated adhesion decreases this oriented axon formation in vitro, and disrupts the axon–dendrite polarization in vivo. Furthermore, the RGC–neuron interaction induces the polarized distribution of active RhoA at the contacting neurite and active Rac1 at the opposite neurite. Inhibition of Rho–Rho-kinase signaling in a neuron impairs the oriented axon formation in vitro, and prevents axon–dendrite polarization in vivo. Collectively, these results suggest that the N-cadherin-mediated radial glia–neuron interaction determines the contacting neurite as the leading process for radial glia-guided neuronal migration and directs axon formation to the opposite side acting through the Rho family GTPases.

本文言語English
ページ(範囲)14517-14532
ページ数16
ジャーナルJournal of Neuroscience
35
43
DOI
出版ステータスPublished - 28-10-2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)

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