Rapid and sensitive vasopressin heteronuclear RNA responses to changes in plasma osmolality

Hiroshi Arima, K. Kondo, S. Kakiya, H. Nagasaki, H. Yokoi, Y. Yambe, T. Murase, Y. Iwasaki, Y. Oiso

研究成果: Article査読

69 被引用数 (Scopus)

抄録

The plasma concentration of arginine vasopressin (AVP) is closely regulated by plasma osmolality. In this study, we used intronic in-situ hybridization to investigate the transcriptional activity of the AVP gene in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) after intraperitoneal (i.p.) injection of hypertonic saline inducing small changes in plasma osmolality in rats. After ip injection of 900 mOsm/kg saline (2% BW), plasma [Na] reached the highest level at 10 min (900 mOsm/kg, 146 ± 1 mEq/l; 290 mOsm/kg, 139 ± 1 mEq/l, P < 0.01) and maintained that level until 30 min. The expression of AVP heteronuclear (hn) RNA in the SON and PVN increased significantly as early as 10 min and peaked 30 min after ip injection of the hypertonic saline. AVP hnRNA expression showed a significant increase even after a 2 mEq/L elevation in plasma [Na] concentration, and increased dose-dependently in response to the concentration of saline injection (290-900 mOsm/kg). These results demonstrate that, similar to AVP secretion, AVP gene transcriptional activity in the SON and PVN is closely regulated by plasma osmolality.

本文言語English
ページ(範囲)337-341
ページ数5
ジャーナルJournal of Neuroendocrinology
11
5
DOI
出版ステータスPublished - 07-05-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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