TY - JOUR
T1 - Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method
AU - Iwata, Seiko
AU - Shibata, Yukiko
AU - Kawada, Jun ichi
AU - Hara, Shinya
AU - Nishiyama, Yukihiro
AU - Morishima, Tsuneo
AU - Ihira, Masaru
AU - Yoshikawa, Tetsushi
AU - Asano, Yoshizo
AU - Kimura, Hiroshi
N1 - Funding Information:
We thank the following people for their contributions to this study: Dr. Yoshihiro Ando (Aichi Children's Health and Medical Center), Dr. Kazuo Nishikawa (Nagoya Ekisaikai Hospital), Dr. Makoto Morita (Nagoya Memorial Hospital), and Dr. Akimasa Ogawa (Anjo Kosei Hospital). This work was supported by grants from the Ministry of Education, Culture, Sport, Science, and Technology of Japan.
PY - 2006/10
Y1 - 2006/10
N2 - Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4% and the specificity was 100%. The sensitivity of the real-time PCR assay was 84.1% and the specificity was 98.4%. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection.
AB - Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4% and the specificity was 100%. The sensitivity of the real-time PCR assay was 84.1% and the specificity was 98.4%. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection.
UR - https://www.scopus.com/pages/publications/33748954455
UR - https://www.scopus.com/pages/publications/33748954455#tab=citedBy
U2 - 10.1016/j.jcv.2006.07.011
DO - 10.1016/j.jcv.2006.07.011
M3 - Article
C2 - 16973412
AN - SCOPUS:33748954455
SN - 1386-6532
VL - 37
SP - 128
EP - 133
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
IS - 2
ER -
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