Rapid detection of herpes simplex virus DNA in cerebrospinal fluid: Comparison between loop-mediated isothermal amplification and real-time PCR

Hiroshi Kimura, Masaru Ihira, Yoshihiro Enomoto, Jun Ichi Kawada, Yoshinori Ito, Tsuneo Morishima, Tetsushi Yoshikawa, Yoshizo Asano

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.

本文言語English
ページ(範囲)181-185
ページ数5
ジャーナルMedical Microbiology and Immunology
194
4
DOI
出版ステータスPublished - 08-2005

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 微生物学(医療)

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