Rapid purification and structural study of DNA topoisomerase I from human burkitt lymphoma raji cells

Motoshi Suzuki, Eishi Takagi, Kiyohide Kojima, Shunji Izuta, Shonen Yoshida

研究成果: Article

1 引用 (Scopus)

抜粋

We have developed a rapid purification method for DNA topoisomerase I from Raji cells, a human Burkitt lymphoma cell line, using ammonium sulfate fractionation followed by chromatography on a Mono S column (FPLC, Pharmacia). By this method, the enzyme could be purified to near homogeneity within one day. Electrophoresis on sodium dodecyl sulfate polyacrylamide gel revealed that the final preparation is mainly composed of a 100-kDa protein. The major enzyme activity sedimented through a glycerol density gradient at 5.7S, accompanied with a minor peak at 8.7S. The former may correspond to the monomer of the 100-kDa polypeptide, and the latter, to its dimeric form. The gel filtration study of the crude extract revealed an active molecular species of 200 kDa, in addition to 100 kDa, and lower molecular weight forms. These results suggest that DNA topoisomerase I is largely in monomeric form, but also has a minor population of the dimeric form.

元の言語English
ページ(範囲)742-744
ページ数3
ジャーナルJournal of Biochemistry
106
発行部数5
DOI
出版物ステータスPublished - 11-1989
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

フィンガープリント Rapid purification and structural study of DNA topoisomerase I from human burkitt lymphoma raji cells' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用