Rapid, simple and simultaneous measurement of kynurenine and tryptophan in plasma by column switching-HPLC method

K. Kawai, H. Ishikawa, K. Ohashi, Y. Itoh, R. Teradaira

研究成果: Article

9 引用 (Scopus)

抄録

We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.

元の言語English
ページ(範囲)415-419
ページ数5
ジャーナルInternational Congress Series
1304
DOI
出版物ステータスPublished - 01-11-2007

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Kynurenine
Tryptophan
High Pressure Liquid Chromatography
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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AU - Kawai, K.

AU - Ishikawa, H.

AU - Ohashi, K.

AU - Itoh, Y.

AU - Teradaira, R.

PY - 2007/11/1

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AB - We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.

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