TY - GEN
T1 - Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices
AU - Sun, Miaomiao
AU - Kasama, Toshihiro
AU - Kaji, Noritada
AU - Akiyama, Shin Ichi
AU - Yuzawa, Yukio
AU - Matsuo, Seiichi
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2012
Y1 - 2012
N2 - We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.
AB - We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.
UR - http://www.scopus.com/inward/record.url?scp=84901796095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901796095&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901796095
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 812
EP - 814
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -
