Rbm38 reduces the transcription elongation defect of the SMEK2 gene caused by splicing deficiency

Shintaro Muraoka, Kazuhiro Fukumura, Megumi Hayashi, Naoyuki Kataoka, Akila Mayeda, Daisuke Kaida

研究成果: Article査読

抄録

Pre-mRNA splicing is an essential mechanism for ensuring integrity of the transcriptome in eukaryotes. Therefore, splicing deficiency might cause a decrease in functional proteins and the production of nonfunctional, aberrant proteins. To prevent the production of such aberrant proteins, eukaryotic cells have several mRNA quality control mechanisms. In addition to the known mechanisms, we previously found that transcription elongation is attenuated to prevent the accumulation of pre-mRNA under splicing-deficient conditions. However, the detailed molecular mechanism behind the defect in transcription elongation remains unknown. Here, we showed that the RNA binding protein Rbm38 reduced the transcription elongation defect of the SMEK2 gene caused by splicing deficiency. This reduction was shown to require the N-and C-terminal regions of Rbm38, along with an important role being played by the RNA-recognition motif of Rbm38. These findings advance our understanding of the molecular mechanism of the transcription elongation defect caused by splicing deficiency.

本文言語English
論文番号8799
ページ(範囲)1-13
ページ数13
ジャーナルInternational journal of molecular sciences
21
22
DOI
出版ステータスPublished - 02-11-2020

All Science Journal Classification (ASJC) codes

  • 触媒
  • 分子生物学
  • 分光学
  • コンピュータ サイエンスの応用
  • 物理化学および理論化学
  • 有機化学
  • 無機化学

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