Re-examination by improved reverse transcriptase assay of DNA polymerase in platelets from myelodysplastic disease patients

S. Yoshida, H. Yamada, T. Sakurai, M. Suzuki, K. Kojima

研究成果: Article査読

3 被引用数 (Scopus)

抄録

A number of reports have suggested that platelets from polycythemia vera patients contain reverse transcriptase activity that might be correlated with C-type retrovirus-like particles. As described herein, we devised a new assay method for reverse transcriptase activity using MS-2 phage RNA hybridized with synthetic oligodeoxynucleotide (18-mer) as a template/primer. Using this new, sensitive assay method, we examined the platelet enzyme. By the conventional assay method using poly(rA)-oligo (dT), extracts of platelets showed a considerable amount of incorporation. However, by the new assay method using MS-2 RNA, no incorporation was observed. The poly(rA)-oligo(dT)-dependent activity was purified on Mono Q column, and it was shown that this activity coincided with that of DNA polymerase γ.

本文言語English
ページ(範囲)1133-1141
ページ数9
ジャーナルBiochemistry International
19
5
出版ステータスPublished - 1989
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学

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