Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Takeshi Onoue; Motomitsu Goto; Takashi Tominaga; Mariko Sugiyama; Taku Tsunekawa; Daisuke Hagiwara; Ryoichi Banno; Hidetaka Suga; Yoshihisa Sugimura; Hiroshi Arima

doi:10.1016/j.neulet.2016.03.011

Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Takeshi Onoue, Motomitsu Goto, Takashi Tominaga, Mariko Sugiyama, Taku Tsunekawa, Daisuke Hagiwara, Ryoichi Banno, Hidetaka Suga, Yoshihisa Sugimura, Hiroshi Arima

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

9 被引用数 (Scopus)

抄録

In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H₂O₂ increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

本文言語	英語
ページ（範囲）	1-7
ページ数	7
ジャーナル	Neuroscience Letters
巻	619
DOI	https://doi.org/10.1016/j.neulet.2016.03.011
出版ステータス	出版済み - 21-04-2016
外部発表	はい

All Science Journal Classification (ASJC) codes

神経科学一般

文献へのアクセス

10.1016/j.neulet.2016.03.011

他のファイルとリンク

引用スタイル

@article{fc80c2b711b348f5b70db12bcdcf07c8,

title = "Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus",

abstract = "In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.",

author = "Takeshi Onoue and Motomitsu Goto and Takashi Tominaga and Mariko Sugiyama and Taku Tsunekawa and Daisuke Hagiwara and Ryoichi Banno and Hidetaka Suga and Yoshihisa Sugimura and Hiroshi Arima",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Ireland Ltd.",

year = "2016",

month = apr,

day = "21",

doi = "10.1016/j.neulet.2016.03.011",

language = "English",

volume = "619",

pages = "1--7",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

AU - Onoue, Takeshi

AU - Goto, Motomitsu

AU - Tominaga, Takashi

AU - Sugiyama, Mariko

AU - Tsunekawa, Taku

AU - Hagiwara, Daisuke

AU - Banno, Ryoichi

AU - Suga, Hidetaka

AU - Sugimura, Yoshihisa

AU - Arima, Hiroshi

PY - 2016/4/21

Y1 - 2016/4/21

N2 - In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

AB - In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

UR - http://www.scopus.com/inward/record.url?scp=84960927921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960927921&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2016.03.011

DO - 10.1016/j.neulet.2016.03.011

M3 - Article

C2 - 26968348

AN - SCOPUS:84960927921

SN - 0304-3940

VL - 619

SP - 1

EP - 7

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

抄録

All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル