Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro

Yasuyo Shimomura, Mika Suga, Naohide Kuriyama, Tomoyuki Nakamura, Toshikazu Sakai, Yu Kato, Yoshitaka Hara, Chizuru Yamashita, Hiroshi Nagasaki, Masao Kaneki, Osamu Nishida

研究成果: Letter

9 引用 (Scopus)

抄録

The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 % of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.

元の言語English
記事番号48
ジャーナルJournal of Intensive Care
4
発行部数1
DOI
出版物ステータスPublished - 22-07-2016

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Lipopolysaccharides
Blood Platelets
Neutrophils
Coculture Techniques
In Vitro Techniques
human THBD protein
Extracellular Traps
Confocal Microscopy

All Science Journal Classification (ASJC) codes

  • Critical Care and Intensive Care Medicine

これを引用

Shimomura, Yasuyo ; Suga, Mika ; Kuriyama, Naohide ; Nakamura, Tomoyuki ; Sakai, Toshikazu ; Kato, Yu ; Hara, Yoshitaka ; Yamashita, Chizuru ; Nagasaki, Hiroshi ; Kaneki, Masao ; Nishida, Osamu. / Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro. :: Journal of Intensive Care. 2016 ; 巻 4, 番号 1.
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title = "Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro",
abstract = "The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 {\%} of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.",
author = "Yasuyo Shimomura and Mika Suga and Naohide Kuriyama and Tomoyuki Nakamura and Toshikazu Sakai and Yu Kato and Yoshitaka Hara and Chizuru Yamashita and Hiroshi Nagasaki and Masao Kaneki and Osamu Nishida",
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Shimomura, Y, Suga, M, Kuriyama, N, Nakamura, T, Sakai, T, Kato, Y, Hara, Y, Yamashita, C, Nagasaki, H, Kaneki, M & Nishida, O 2016, 'Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro', Journal of Intensive Care, 巻. 4, 番号 1, 48. https://doi.org/10.1186/s40560-016-0177-9

Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro. / Shimomura, Yasuyo; Suga, Mika; Kuriyama, Naohide; Nakamura, Tomoyuki; Sakai, Toshikazu; Kato, Yu; Hara, Yoshitaka; Yamashita, Chizuru; Nagasaki, Hiroshi; Kaneki, Masao; Nishida, Osamu.

:: Journal of Intensive Care, 巻 4, 番号 1, 48, 22.07.2016.

研究成果: Letter

TY - JOUR

T1 - Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro

AU - Shimomura, Yasuyo

AU - Suga, Mika

AU - Kuriyama, Naohide

AU - Nakamura, Tomoyuki

AU - Sakai, Toshikazu

AU - Kato, Yu

AU - Hara, Yoshitaka

AU - Yamashita, Chizuru

AU - Nagasaki, Hiroshi

AU - Kaneki, Masao

AU - Nishida, Osamu

PY - 2016/7/22

Y1 - 2016/7/22

N2 - The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 % of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.

AB - The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 % of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.

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DO - 10.1186/s40560-016-0177-9

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VL - 4

JO - Journal of Intensive Care

JF - Journal of Intensive Care

SN - 2052-0492

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