Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8+ T cells

Raul Vizcardo, Kyoko Masuda, Daisuke Yamada, Tomokatsu Ikawa, Kanako Shimizu, Shin Ichiro Fujii, Haruhiko Koseki, Hiroshi Kawamoto

研究成果: Article査読

173 被引用数 (Scopus)

抄録

Antigen-specific T cells represent a potential therapeutic avenue for a variety of conditions, but current approaches for generating such cells for therapeutic purposes are limited. In this study, we established iPSCs from mature cytotoxic T cells specific for the melanoma epitope MART-1. When cocultured with OP9/DLL1 cells, these iPSCs efficiently generated TCRβ+CD4+CD8+ double positive (DP) cells expressing a T cell receptor (TCR) specific for the MART-1 epitope. Stimulation of these DP cells with anti-CD3 antibody generated a large number of CD8 + T cells, and more than 90% of the resulting cells were specific for the original MART-1 epitope. Stimulation of the CD8+ T cells with MART-1 antigen-presenting cells led to the secretion of IFNγ, demonstrating their specific reactivity. The present study therefore illustrates an approach for cloning and expanding functional antigen-specific CD8 + T cells that might be applicable in cell-based therapy of cancer.

本文言語English
ページ(範囲)31-36
ページ数6
ジャーナルCell Stem Cell
12
1
DOI
出版ステータスPublished - 03-01-2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 遺伝学
  • 細胞生物学

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