抄録
Antigen-specific T cells represent a potential therapeutic avenue for a variety of conditions, but current approaches for generating such cells for therapeutic purposes are limited. In this study, we established iPSCs from mature cytotoxic T cells specific for the melanoma epitope MART-1. When cocultured with OP9/DLL1 cells, these iPSCs efficiently generated TCRβ+CD4+CD8+ double positive (DP) cells expressing a T cell receptor (TCR) specific for the MART-1 epitope. Stimulation of these DP cells with anti-CD3 antibody generated a large number of CD8 + T cells, and more than 90% of the resulting cells were specific for the original MART-1 epitope. Stimulation of the CD8+ T cells with MART-1 antigen-presenting cells led to the secretion of IFNγ, demonstrating their specific reactivity. The present study therefore illustrates an approach for cloning and expanding functional antigen-specific CD8 + T cells that might be applicable in cell-based therapy of cancer.
本文言語 | 英語 |
---|---|
ページ(範囲) | 31-36 |
ページ数 | 6 |
ジャーナル | Cell Stem Cell |
巻 | 12 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 03-01-2013 |
All Science Journal Classification (ASJC) codes
- 分子医療
- 遺伝学
- 細胞生物学