TY - JOUR
T1 - Regulation of myosin phosphatase through phosphorylation of the myosin- binding subunit in platelet activation
AU - Nakai, Keiji
AU - Suzuki, Yoshinori
AU - Kihira, Hisakazu
AU - Wada, Hideo
AU - Fujioka, Masanori
AU - Ito, Masaaki
AU - Nakano, Takeshi
AU - Kaibuchi, Kozo
AU - Shiku, Hiroshi
AU - Nishikawa, Masakatsu
PY - 1997/11/15
Y1 - 1997/11/15
N2 - Human platelets were found to contain myosin phosphatase consisting of a 38-kD catalytic subunit of protein phosphatase type 18, a 130-kD myosin- binding subunit (MBS) and a 20-kD subunit, all of which cross-reacted with antibodies against these subunits of smooth muscle myosin phosphatase. Anti- MBS antibody coimmunoprecipitated RhoA and Rho-kinase of human platelets. Platelets MBS is a substrate for Rho-kinase and phosphorylation of MBS decreases the activity of myosin phosphatase. Treatment of intact platelets with 9,11-epithio-11,12-methano-thromboxane A2 led to a dramatic increase in phosphorylation of MBS and a significant decrease in the activity of myosin phosphatase. These findings suggest a putative mechanism for agonist-induced regulation of myosin phosphatase activity in platelets.
AB - Human platelets were found to contain myosin phosphatase consisting of a 38-kD catalytic subunit of protein phosphatase type 18, a 130-kD myosin- binding subunit (MBS) and a 20-kD subunit, all of which cross-reacted with antibodies against these subunits of smooth muscle myosin phosphatase. Anti- MBS antibody coimmunoprecipitated RhoA and Rho-kinase of human platelets. Platelets MBS is a substrate for Rho-kinase and phosphorylation of MBS decreases the activity of myosin phosphatase. Treatment of intact platelets with 9,11-epithio-11,12-methano-thromboxane A2 led to a dramatic increase in phosphorylation of MBS and a significant decrease in the activity of myosin phosphatase. These findings suggest a putative mechanism for agonist-induced regulation of myosin phosphatase activity in platelets.
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U2 - 10.1182/blood.v90.10.3936
DO - 10.1182/blood.v90.10.3936
M3 - Article
C2 - 9354661
AN - SCOPUS:0030775882
SN - 0006-4971
VL - 90
SP - 3936
EP - 3942
JO - Blood
JF - Blood
IS - 10
ER -