Regulation of proliferating cell nuclear antigen ubiquitination in mammalian cells

Atsuko Niimi, Stephanie Brown, Simone Sabbioneda, Patricia L. Kannouche, Andrew Scott, Akira Yasui, Catherine M. Green, Alan R. Lehmann

研究成果: ジャーナルへの寄稿学術論文査読

148 被引用数 (Scopus)


After exposure to DNA-damaging agents that block the progress of the replication fork, monoubiquitination of proliferating cell nuclear antigen (PCNA) mediates the switch from replicative to translesion synthesis DNA polymerases. We show that in human cells, PCNA is monoubiquitinated in response to methyl methane-sulfonate and mitomycin C, as well as UV light, albeit with different kinetics, but not in response to bleomycin or camptothecin. Cyclobutane pyrimidine dimers are responsible for most of the PCNA ubiquitination events after UV-irradiation. Failure to ubiquitinate PCNA results in substantial sensitivity to UV and methyl methanesulfonate, but not to camptothecin or bleomycin. PCNA ubiquitination depends on Replication Protein A (RPA), but is independent of ATR-mediated checkpoint activation. After UV-irradiation, there is a temporal correlation between the disappearance of the deubiquitinating enzyme USP1 and the presence of PCNA ubiquitination, but this correlation was not found after chemical mutagen treatment. By using cells expressing photolyases, we are able to remove the UV lesions, and we show that PCNA ubiquitination persists for many hours after the damage has been removed. We present a model of translesion synthesis behind the replication fork to explain the persistence of ubiquitinated PCNA.

ジャーナルProceedings of the National Academy of Sciences of the United States of America
出版ステータス出版済み - 21-10-2008

All Science Journal Classification (ASJC) codes

  • 一般


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