Regulation of the Early Subnormal Retinal Oxygenation Response in Experimental Diabetes by Inducible Nitric Oxide Synthase

Bruce A. Berkowitz, Hongmei Luan, Rita R. Gupta, Daniel Pacheco, Andres Seidner, Robin Roberts, Jessica Liggett, Deborah L. Knoerzer, Jane R. Connor, Yunpeng Du, Timothy S. Kern, Yasuki Ito

研究成果: ジャーナルへの寄稿学術論文査読

40 被引用数 (Scopus)

抄録

We aimed to test the hypothesis that the inducible form of nitric oxide synthase (iNOS) contributes to the development of an early subnormal retinal oxygenation response in preclinical models of diabetic retinopathy. In urethane anesthetized Sprague Dawley rats or C57BL/6 mice, functional magnetic resonance imaging was used to noninvasively measure the change in retinal oxygen tension (ΔPO2) during a carbogen-inhalation challenge. In the rat experiments, the retinal ΔPO2 of the following groups were compared: control rats (n = 9), 3-month diabetic rats (n = 5), and 3-month diabetic rats treated orally with L-N(6)-(1-iminoethyl)lysine 5-tetrazole amide, a prodrug of an inhibitor of iNOS (n = 6). In addition, the retinal ΔPO2 of the following mouse groups were compared: C57BL/6 mice (n = 20), C57BL/6-Nos2tm1Lau mice (n = 10), 4-month diabetic mice (n = 13), and 4-month diabetic knockout mice (n = 6). Only the ΔPO 2 of the superior hemiretina of the diabetic rat and mice groups were significantly subnormal (P < 0.05). The superior ΔPO2 of the diabetic rats treated with the prodrug was not significantly (P > 0.05) different from their respective normal controls. In the mice experiments, the superior retinal ΔPO2 of the iNOS null mice was not statistically different (P > 0.05) from that of normal control mice. iNOS is required for the development of an early subnormal ΔPO2 in experimental diabetic retinopathy.

本文言語英語
ページ(範囲)173-178
ページ数6
ジャーナルDiabetes
53
1
DOI
出版ステータス出版済み - 01-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 内科学
  • 内分泌学、糖尿病および代謝内科学

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