Regulation of Translesion Synthesis DNA Polymerase η by Monoubiquitination

Marzena Bienko, Catherine M. Green, Simone Sabbioneda, Nicola Crosetto, Ivan Matic, Richard G. Hibbert, Tihana Begovic, Atsuko Niimi, Matthias Mann, Alan R. Lehmann, Ivan Dikic

研究成果: ジャーナルへの寄稿学術論文査読

136 被引用数 (Scopus)


DNA polymerase η is a Y family polymerase involved in translesion synthesis (TLS). Its action is initiated by simultaneous interaction between the PIP box in pol η and PCNA and between the UBZ in pol η and monoubiquitin attached to PCNA. Whereas monoubiquitination of PCNA is required for its interaction with pol η during TLS, we now show that monoubiquitination of pol η inhibits this interaction, preventing its functions in undamaged cells. Identification of monoubiquitination sites within pol η nuclear localization signal (NLS) led to the discovery that pol η NLS directly contacts PCNA, forming an extended pol η-PCNA interaction surface. We name this the PCNA-interacting region (PIR) and show that its monoubiquitination is downregulated by various DNA-damaging agents. We propose that this mechanism ensures optimal availability of nonubiquitinated, TLS-competent pol η after DNA damage. Our work shows how monoubiquitination can either positively or negatively regulate the assembly of a protein complex, depending on which substrates are targeted by ubiquitin.

ジャーナルMolecular Cell
出版ステータス出版済み - 12-02-2010

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞生物学


「Regulation of Translesion Synthesis DNA Polymerase η by Monoubiquitination」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。