Remodeling of glycans using glycosyltransferase genes

Yuko Naito-Matsui, Hiromu Takematsu

研究成果: Chapter

抄録

Remodeling of glycans on the cell surface is an essential technique to analyze cellular function of lectin-glycan ligand interaction. Here we describe the methods to identify the responsible enzyme (glycosyltransferase) regulating the expression of the glycan of interest and to modulate the glycan expression by overexpressing the glycosyltransferase gene. For the identification of the responsible enzyme, we introduce a new method, CIRES (correlation index-based responsible-enzyme gene screening), that consists of statistical comparison of glycan expression profile obtained by flow cytometry and gene expression profile obtained by DNA microarray.

本文言語English
ホスト出版物のタイトルLectins
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ379-387
ページ数9
ISBN(印刷版)9781493912919
DOI
出版ステータスPublished - 2014
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1200
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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