TY - JOUR
T1 - Ret-Dependent Cell Rearrangements in the Wolffian Duct Epithelium Initiate Ureteric Bud Morphogenesis
AU - Chi, Xuan
AU - Michos, Odyssé
AU - Shakya, Reena
AU - Riccio, Paul
AU - Enomoto, Hideki
AU - Licht, Jonathan D.
AU - Asai, Naoya
AU - Takahashi, Masahide
AU - Ohgami, Nobutaka
AU - Kato, Masashi
AU - Mendelsohn, Cathy
AU - Costantini, Frank
N1 - Funding Information:
This work was supported by National Institutes of Health grants to F.C (DK55388 and DK75578) and J.L. (CA59998) and fellowships from the American Heart Association (X.C). and National Kidney Foundation (O.M.). We thank Andrew McMahon for Hoxb7/Cre mice, Vassilis Pachnis for Ret tm2(RET)Vpa mice, Chyuan-Sheng Lin and Zaiqi Wu for help with ES cell and chimera generation, and Qais Al-Awqati and Doris Herzlinger for helpful discussions.
PY - 2009/8/18
Y1 - 2009/8/18
N2 - While the genetic control of renal branching morphogenesis has been extensively described, the cellular basis of this process remains obscure. GDNF/RET signaling is required for ureter and kidney development, and cells lacking Ret are excluded from the tips of the branching ureteric bud in chimeric kidneys. Here, we find that this exclusion results from earlier Ret-dependent cell rearrangements in the caudal Wolffian duct, which generate a specialized epithelial domain that later emerges as the tip of the primary ureteric bud. By juxtaposing cells with elevated or reduced RET activity, we find that Wolffian duct cells compete, based on RET signaling levels, to contribute to this domain. At the same time, the caudal Wolffian duct transiently converts from a simple to a pseudostratified epithelium, a process that does not require Ret. Thus, both Ret-dependent cell movements and Ret-independent changes in the Wolffian duct epithelium contribute to ureteric bud formation.
AB - While the genetic control of renal branching morphogenesis has been extensively described, the cellular basis of this process remains obscure. GDNF/RET signaling is required for ureter and kidney development, and cells lacking Ret are excluded from the tips of the branching ureteric bud in chimeric kidneys. Here, we find that this exclusion results from earlier Ret-dependent cell rearrangements in the caudal Wolffian duct, which generate a specialized epithelial domain that later emerges as the tip of the primary ureteric bud. By juxtaposing cells with elevated or reduced RET activity, we find that Wolffian duct cells compete, based on RET signaling levels, to contribute to this domain. At the same time, the caudal Wolffian duct transiently converts from a simple to a pseudostratified epithelium, a process that does not require Ret. Thus, both Ret-dependent cell movements and Ret-independent changes in the Wolffian duct epithelium contribute to ureteric bud formation.
KW - DEVBIO
UR - https://www.scopus.com/pages/publications/68349120381
UR - https://www.scopus.com/pages/publications/68349120381#tab=citedBy
U2 - 10.1016/j.devcel.2009.07.013
DO - 10.1016/j.devcel.2009.07.013
M3 - Article
C2 - 19686681
AN - SCOPUS:68349120381
SN - 1534-5807
VL - 17
SP - 199
EP - 209
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -