Retinal phototoxicity in a novel murine model of intraocular lens implantation.

Toshihide Kurihara, Masahiro Omoto, Kousuke Noda, Mari Ebinuma, Shunsuke Kubota, Haruna Koizumi, Satoru Yoshida, Yoko Ozawa, Shigeto Shimmura, Susumu Ishida, Kazuo Tsubota

研究成果: Article査読

18 被引用数 (Scopus)

抄録

PURPOSE: To establish a novel murine intraocular lens (IOL) implantation model to study the protective effects of colored-IOLs against retinal phototoxicity. METHODS: Two-millimeter diameter IOL buttons were created from IOLs for clinical use. Extra-capsular crystalline lens extraction and IOL implantation were performed in BALB/c mice using a technique similar to human cataract surgery. For light exposure experiments, mice were exposed to 5,000 LUX of white light for 24 h on the day after surgery. To investigate the protective effects of yellow IOL against light exposure, ERG measurements were conducted in vivo, followed by TdT-mediated dUTP Nick-End Labeling (TUNEL) and outer nuclear layer (ONL) thickness measurement of retinal tissue in yellow or clear IOL-implanted mice and control mice without surgery. RESULTS: IOLs were successfully implanted in all animals, and IOL buttons without haptics were well stabilized in the capsular bag. Murine eyes developed posterior capsule opacification (PCO) after IOL implantation by postoperative day 5 at the latest. In contrast to the clear IOL-implanted animals stimulated by light exposure, the yellow IOL-implanted animals had significantly reduced numbers of TUNEL-positive cells and retained thickness of the ONL. The ERG showed that yellow IOL implantation prevents a decrease of amplitude in both the a-wave and b-wave compared with clear IOL implantation. CONCLUSIONS: We established a new animal model of IOL implantation and demonstrated the protective effects of colored-IOL against retinal phototoxicity after cataract surgery.

本文言語English
ページ(範囲)2751-2761
ページ数11
ジャーナルMolecular vision
15
出版ステータスPublished - 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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