TY - JOUR
T1 - Reverse genetics system for human rotaviruses
AU - Komoto, Satoshi
AU - Fukuda, Saori
AU - Murata, Takayuki
AU - Taniguchi, Koki
N1 - Publisher Copyright:
© 2020 The Societies and John Wiley & Sons Australia, Ltd
PY - 2020/6/1
Y1 - 2020/6/1
N2 - A reverse genetics technology is an incredibly useful technique both for a proper understanding of different aspects of virus biology and for the generation of complementary DNA (cDNA)-derived infectious viruses, which can act as safe and effective vaccines and viral vectors. Rotaviruses (RVAs), especially human RVAs (HuRVAs), had been very refractory to this technology until very recently. Here, we describe the historical background of the development of a long-awaited HuRVA reverse genetics system, culminating in the generation of replicative HuRVAs entirely from cloned cDNAs.
AB - A reverse genetics technology is an incredibly useful technique both for a proper understanding of different aspects of virus biology and for the generation of complementary DNA (cDNA)-derived infectious viruses, which can act as safe and effective vaccines and viral vectors. Rotaviruses (RVAs), especially human RVAs (HuRVAs), had been very refractory to this technology until very recently. Here, we describe the historical background of the development of a long-awaited HuRVA reverse genetics system, culminating in the generation of replicative HuRVAs entirely from cloned cDNAs.
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U2 - 10.1111/1348-0421.12795
DO - 10.1111/1348-0421.12795
M3 - Review article
C2 - 32293752
AN - SCOPUS:85084218979
SN - 0385-5600
VL - 64
SP - 401
EP - 406
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 6
ER -
