RFP is a DNA binding protein associated with the nuclear matrix

Takeshi Isomura, Keiko Tamiya-koizumi, Motoshi Suzuki, Shonen Yoshida, Masahiko Taniguchi, Mutsushi Matsuyama, Takeo Ishigaki, Sadayuki Sakuma, Masahide Takahashi

研究成果: Article

47 引用 (Scopus)

抄録

We reported that the RFP gene encodes a protein with putative zinc finger domains and was involved In the activation of the ret proto-oncogene. To further characterize the RFP protein, we developed a polyclonal antibody against the product synthesized from a fragment of the RFP cDNA expressed in Escherichia coll. Western blot analysis showed that RFP was identified as a 58 kDa protein In cell lysates from four human and rodent cell lines and from mouse test is. In addition, a unique 68 kDa protein was detected in the testis. Using AH7974 (rat ascites hepatoma) and Raji (human Burkttt lymphoma) cells, we demonstrated strong association of RFP with the nuclear matrix. Furthermore, RFP solubilized from the nuclear matrix had DNA-bindlng activity although It appears to bind more preferentially to double-stranded DNA than to single-stranded DNA. These results thus suggest that RFP may play a role in molecular processes which occur in the nuclear matrix.

元の言語English
ページ(範囲)5305-5310
ページ数6
ジャーナルNucleic Acids Research
20
発行部数20
DOI
出版物ステータスPublished - 25-10-1992

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Nuclear Matrix
DNA-Binding Proteins
Proteins
Escherichia
Proto-Oncogenes
Single-Stranded DNA
DNA
Zinc Fingers
Ascites
Testis
Hepatocellular Carcinoma
Rodentia
Lymphoma
Complementary DNA
Western Blotting
Cell Line
Antibodies
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Isomura, T., Tamiya-koizumi, K., Suzuki, M., Yoshida, S., Taniguchi, M., Matsuyama, M., ... Takahashi, M. (1992). RFP is a DNA binding protein associated with the nuclear matrix. Nucleic Acids Research, 20(20), 5305-5310. https://doi.org/10.1093/nar/20.20.5305
Isomura, Takeshi ; Tamiya-koizumi, Keiko ; Suzuki, Motoshi ; Yoshida, Shonen ; Taniguchi, Masahiko ; Matsuyama, Mutsushi ; Ishigaki, Takeo ; Sakuma, Sadayuki ; Takahashi, Masahide. / RFP is a DNA binding protein associated with the nuclear matrix. :: Nucleic Acids Research. 1992 ; 巻 20, 番号 20. pp. 5305-5310.
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Isomura, T, Tamiya-koizumi, K, Suzuki, M, Yoshida, S, Taniguchi, M, Matsuyama, M, Ishigaki, T, Sakuma, S & Takahashi, M 1992, 'RFP is a DNA binding protein associated with the nuclear matrix', Nucleic Acids Research, 巻. 20, 番号 20, pp. 5305-5310. https://doi.org/10.1093/nar/20.20.5305

RFP is a DNA binding protein associated with the nuclear matrix. / Isomura, Takeshi; Tamiya-koizumi, Keiko; Suzuki, Motoshi; Yoshida, Shonen; Taniguchi, Masahiko; Matsuyama, Mutsushi; Ishigaki, Takeo; Sakuma, Sadayuki; Takahashi, Masahide.

:: Nucleic Acids Research, 巻 20, 番号 20, 25.10.1992, p. 5305-5310.

研究成果: Article

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AU - Tamiya-koizumi, Keiko

AU - Suzuki, Motoshi

AU - Yoshida, Shonen

AU - Taniguchi, Masahiko

AU - Matsuyama, Mutsushi

AU - Ishigaki, Takeo

AU - Sakuma, Sadayuki

AU - Takahashi, Masahide

PY - 1992/10/25

Y1 - 1992/10/25

N2 - We reported that the RFP gene encodes a protein with putative zinc finger domains and was involved In the activation of the ret proto-oncogene. To further characterize the RFP protein, we developed a polyclonal antibody against the product synthesized from a fragment of the RFP cDNA expressed in Escherichia coll. Western blot analysis showed that RFP was identified as a 58 kDa protein In cell lysates from four human and rodent cell lines and from mouse test is. In addition, a unique 68 kDa protein was detected in the testis. Using AH7974 (rat ascites hepatoma) and Raji (human Burkttt lymphoma) cells, we demonstrated strong association of RFP with the nuclear matrix. Furthermore, RFP solubilized from the nuclear matrix had DNA-bindlng activity although It appears to bind more preferentially to double-stranded DNA than to single-stranded DNA. These results thus suggest that RFP may play a role in molecular processes which occur in the nuclear matrix.

AB - We reported that the RFP gene encodes a protein with putative zinc finger domains and was involved In the activation of the ret proto-oncogene. To further characterize the RFP protein, we developed a polyclonal antibody against the product synthesized from a fragment of the RFP cDNA expressed in Escherichia coll. Western blot analysis showed that RFP was identified as a 58 kDa protein In cell lysates from four human and rodent cell lines and from mouse test is. In addition, a unique 68 kDa protein was detected in the testis. Using AH7974 (rat ascites hepatoma) and Raji (human Burkttt lymphoma) cells, we demonstrated strong association of RFP with the nuclear matrix. Furthermore, RFP solubilized from the nuclear matrix had DNA-bindlng activity although It appears to bind more preferentially to double-stranded DNA than to single-stranded DNA. These results thus suggest that RFP may play a role in molecular processes which occur in the nuclear matrix.

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Isomura T, Tamiya-koizumi K, Suzuki M, Yoshida S, Taniguchi M, Matsuyama M その他. RFP is a DNA binding protein associated with the nuclear matrix. Nucleic Acids Research. 1992 10 25;20(20):5305-5310. https://doi.org/10.1093/nar/20.20.5305