Rho-associated kinase phosphorylates desmin, the myogenic intermediate filament protein, at unique amino-terminal sites

Hiroyasu Inada, Hidemasa Goto, Kazushi Tanabe, Yoshimi Nishi, Kozo Kaibuchi, Masaki Inagaki

研究成果: Article査読

28 被引用数 (Scopus)

抄録

We obtained evidence that Rho-associated kinase (Rho-kinase) phosphorylates desmin, the myogenic intermediate filament protein, with approximately 2 mol phosphate per mole of desmin in vitro. Desmin phosphorylated by Rho-kinase lost the potential to form 10-nm filaments. Thr-16, Thr-75, and Thr-76 on desmin proved to be the major phosphorylation sites for Rho-kinase. All these sites are located within the head domain and are different from the reported phosphorylation sites of protein kinase A, protein kinase C, and cdc2 kinase. We are entertaining the notion that Rho-kinase may regulate filament structures of desmin by site-specific phosphorylation.

本文言語English
ページ(範囲)21-25
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
253
1
DOI
出版ステータスPublished - 09-12-1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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