TY - JOUR
T1 - Role of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in regulation of E-Cadherin-mediated cell-cell adhesion
AU - Kuroda, Shinya
AU - Fukata, Masaki
AU - Nakagawa, Masato
AU - Fujii, Katsuhiko
AU - Nakamura, Tomoko
AU - Ookubo, Tadashi
AU - Izawa, Ichiro
AU - Nagase, Takahiro
AU - Nomura, Nobuo
AU - Tani, Hideki
AU - Shoji, Ikuo
AU - Matsuura, Yoshiharu
AU - Yonehara, Shin
AU - Kaibuchi, Kozo
PY - 1998/8/7
Y1 - 1998/8/7
N2 - The small guanosine triphosphatases (GTPases) Cdc42 and Rac1 regulate E- cadherin-mediated cell-cell adhesion. IQGAP1, a target of Cdc42 and Rac1, was localized with E-cadherin and β-catenin at sites of cell-cell contact in mouse L fibroblasts expressing E-cadherin (EL cells), and interacted with E- cadherin and β-catenin both in vivo and in vitro. IQGAP1 induced the dissociation of α-catenin from a cadherin-catenin complex in vitro and in vivo. Overexpression of IQGAP1 in EL cells, but not in L cells expressing an E-cadherin-α-catenin chimeric protein, resulted in a decrease in E-cadherin- mediated cell-cell, adhesive activity. Thus, IQGAP1, acting downstream of Cdc42 and Rac1, appears to regulate cell-cell adhesion through the cadherin- catenin pathway.
AB - The small guanosine triphosphatases (GTPases) Cdc42 and Rac1 regulate E- cadherin-mediated cell-cell adhesion. IQGAP1, a target of Cdc42 and Rac1, was localized with E-cadherin and β-catenin at sites of cell-cell contact in mouse L fibroblasts expressing E-cadherin (EL cells), and interacted with E- cadherin and β-catenin both in vivo and in vitro. IQGAP1 induced the dissociation of α-catenin from a cadherin-catenin complex in vitro and in vivo. Overexpression of IQGAP1 in EL cells, but not in L cells expressing an E-cadherin-α-catenin chimeric protein, resulted in a decrease in E-cadherin- mediated cell-cell, adhesive activity. Thus, IQGAP1, acting downstream of Cdc42 and Rac1, appears to regulate cell-cell adhesion through the cadherin- catenin pathway.
UR - http://www.scopus.com/inward/record.url?scp=0032493903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032493903&partnerID=8YFLogxK
U2 - 10.1126/science.281.5378.832
DO - 10.1126/science.281.5378.832
M3 - Article
C2 - 9694656
AN - SCOPUS:0032493903
SN - 0036-8075
VL - 281
SP - 832
EP - 835
JO - Science
JF - Science
IS - 5378
ER -