Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity

Takashi Nishimura, Katsuhiro Kato, Tomoya Yamaguchi, Yuko Fukata, Shigeo Ohno, Kozo Kaibuchi

研究成果: Article査読

226 被引用数 (Scopus)

抄録

Neurons polarize to form elaborate multiple dendrites and one long axon. The establishment and maintenance of axon/dendrite polarity are fundamentally important for neurons. Recent studies have demonstrated that the polarity complex PAR-3-PAR-6-atypical protein kinase C (aPKC) is involved in polarity determination in many tissues and cells. The function of the PAR-3-PAR-6-aPKC protein complex depends on its subcellular localization in polarized cells. PAR-3 accumulates at the tip of growing axons in cultured rat hippocampal neurons, but the molecular mechanism of this localization remains unknown. Here we identify a direct interaction between PAR-3 and KIF3A, a plus-end-directed microtubule motor protein, and show that aPKC can associate with KIF3A through its interaction with PAR-3. The expression of dominant-negative PAR-3 and KIF3A fragments that disrupt PAR-3-KIF3A binding inhibited the accumulation of PAR-3 and aPKC at the tip of the neurites and abolished neuronal polarity. These results suggest that PAR-3 is transported to the distal tip of the axon by KIF3A and that the proper localization of PAR-3 is required to establish neuronal polarity.

本文言語English
ページ(範囲)328-334
ページ数7
ジャーナルNature Cell Biology
6
4
DOI
出版ステータスPublished - 04-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 細胞生物学

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