Roles of POLD4, smallest subunit of DNA polymerase δ, in nuclear structures and genomic stability of human cells

Qin Miao Huang, Tomohiro Akashi, Yuji Masuda, Kenji Kamiya, Takashi Takahashi, Motoshi Suzuki

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Mammalian DNA polymerase δ (pol δ) is essential for DNA replication, though the functions of this smallest subunit of POLD4 have been elusive. We investigated pol δ activities in vitro and found that it was less active in the absence of POLD4, irrespective of the presence of the accessory protein PCNA. shRNA-mediated reduction of POLD4 resulted in a marked decrease in colony formation activity by Calu6, ACC-LC-319, and PC-10 cells. We also found that POLD4 reduction was associated with an increased population of karyomere-like cells, which may be an indication of DNA replication stress and/or DNA damage. The karyomere-like cells retained an ability to progress through the cell cycle, suggesting that POLD4 reduction induces modest genomic instability, while allowing cells to grow until DNA damage reaches an intolerant level. Our results indicate that POLD4 is required for the in vitro pol δ activity, and that it functions in cell proliferation and maintenance of genomic stability of human cells.

本文言語English
ページ(範囲)542-546
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
391
1
DOI
出版ステータスPublished - 01-01-2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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