Route of hepatocyte delivery affects hepatocyte engraftment in the spleen

Hideo Nagata, Masahiro Ito, Chiyoe Shirota, Albert Edge, Timothy C. McCowan, Ira J. Fox

研究成果: Article査読

52 被引用数 (Scopus)

抄録

In laboratory animals, intrasplenic hepatocyte transplantation corrects the physiologic abnormalities associated with decompensated liver disease. The clinical experience with hepatocyte transplantation for cirrhosis has been disappointing when compared with laboratory experience. The route of hepatocyte delivery may influence hepatocyte engraftment and function. Outbred pigs were recipients of allogeneic pig hepatocytes. Donor hepatocytes were isolated by collagenase perfusion and labeled using 5(6)-carboxyfluorescein diacetate succinimidyl-ester (CMFSE). Cells were introduced into pig spleens by infusion through the splenic artery or by direct splenic puncture. Direct intrasplenic injection produced engraftment that was far superior to that obtained using splenic artery infusion. Splenic artery infusion produced a gastric erosion and large areas of splenic necrosis secondary to vascular occlusion with hepatocytes, whereas direct splenic injection was associated with clinically insignificant intraabdominal hemorrhage. The route of hepatocyte delivery may influence hepatocyte engraftment and explain the disparity in efficacy of hepatocyte transplantation between the laboratory and clinic.

本文言語English
ページ(範囲)732-735
ページ数4
ジャーナルTransplantation
76
4
DOI
出版ステータスPublished - 27-08-2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 移植

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