TY - JOUR
T1 - Salivary neopterin and related pterins
T2 - Their comparison to those in plasma and changes in individuals
AU - Ikemoto, Kazuhisa
AU - Sumi-Ichinose, Chiho
AU - Suganuma, Yui
AU - Kano, Taiki
AU - Ihira, Noriko
AU - Nagatsu, Toshiharu
AU - Kondo, Kazunao
N1 - Funding Information:
This work was supported by a grant-in-aid from Fujita Health University.
Publisher Copyright:
© 2021 The Author(s). Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Neopterin (NP), biopterin (BP) and monapterin (MP) exist in saliva. The physiological role of salivary NP as well as the pathophysiological role of increased NP in the immune-activated state has been unclear. Saliva is a characteristic specimen different from other body fluids. In this study, we analysed salivary NP and related pterin compounds, BP and MP and revealed some of its feature. High-performance liquid chromatography (HPLC) analysis of saliva and plasma obtained from 26 volunteers revealed that salivary NP existed mostly in its fully oxidized form. The results suggested that salivary NP as well as BP would mostly originate from the oral cavity, perhaps the salivary glands, and that salivary NP levels might not reflect those in the plasma. We also found that a gender difference existed in correlations between concentrations of salivary total concentrations of NP (tNP) and BP (tBP). HPLC analysis of saliva obtained from 5 volunteers revealed that the concentrations of salivary tNP as well as tBP fluctuated in an irregular fashion in various individuals. MP, a diastereomer of NP, might have come from oral cavity NP itself or its precursor. These results indicated that the nature of salivary NP might be different from that of NP in the blood or urine.
AB - Neopterin (NP), biopterin (BP) and monapterin (MP) exist in saliva. The physiological role of salivary NP as well as the pathophysiological role of increased NP in the immune-activated state has been unclear. Saliva is a characteristic specimen different from other body fluids. In this study, we analysed salivary NP and related pterin compounds, BP and MP and revealed some of its feature. High-performance liquid chromatography (HPLC) analysis of saliva and plasma obtained from 26 volunteers revealed that salivary NP existed mostly in its fully oxidized form. The results suggested that salivary NP as well as BP would mostly originate from the oral cavity, perhaps the salivary glands, and that salivary NP levels might not reflect those in the plasma. We also found that a gender difference existed in correlations between concentrations of salivary total concentrations of NP (tNP) and BP (tBP). HPLC analysis of saliva obtained from 5 volunteers revealed that the concentrations of salivary tNP as well as tBP fluctuated in an irregular fashion in various individuals. MP, a diastereomer of NP, might have come from oral cavity NP itself or its precursor. These results indicated that the nature of salivary NP might be different from that of NP in the blood or urine.
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U2 - 10.1093/jb/mvab076
DO - 10.1093/jb/mvab076
M3 - Article
C2 - 34181024
AN - SCOPUS:85122372164
SN - 0021-924X
VL - 170
SP - 559
EP - 567
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 4
ER -