SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation

Takuya Kuroda, Satoshi Yasuda, Shiori Tachi, Satoko Matsuyama, Shinji Kusakawa, Keiko Tano, Takumi Miura, Akifumi Matsuyama, Yoji Sato

研究成果: Article

抜粋

Clinical applications of human induced pluripotent stem cells (hiPSCs) are expected, but hiPSC lines vary in their differentiation propensity. For efficient selection of hiPSC lines suitable for differentiation into desired cell lineages, here we identify SALL3 as a marker to predict differentiation propensity. SALL3 expression in hiPSCs correlates positively with ectoderm differentiation capacity and negatively with mesoderm/endoderm differentiation capacity. Without affecting self-renewal of hiPSCs, SALL3 knockdown inhibits ectoderm differentiation and conversely enhances mesodermal/endodermal differentiation. Similarly, loss- and gain-of-function studies reveal that SALL3 inversely regulates the differentiation of hiPSCs into cardiomyocytes and neural cells. Mechanistically, SALL3 modulates DNMT3B function and DNA methyltransferase activity, and influences gene body methylation of Wnt signaling-related genes in hiPSCs. These findings suggest that SALL3 switches the differentiation propensity of hiPSCs toward distinct cell lineages by changing the epigenetic profile and serves as a marker for evaluating the hiPSC differentiation propensity.

元の言語English
記事番号2175
ジャーナルNature Communications
10
発行部数1
DOI
出版物ステータスPublished - 01-12-2019

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

これを引用

Kuroda, T., Yasuda, S., Tachi, S., Matsuyama, S., Kusakawa, S., Tano, K., Miura, T., Matsuyama, A., & Sato, Y. (2019). SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation. Nature Communications, 10(1), [2175]. https://doi.org/10.1038/s41467-019-09511-4