Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies

R. Yoshiyuki Osamura, Yoji Suemizu, Shinichi Yoshimura, Sadaaki Hori, Kenichi Inada, Keiichi Watanabe, Yoshikatsu Nakai, Hiroo Imura

研究成果: Article査読

8 被引用数 (Scopus)

抄録

In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pituitary tumor cells with secretory granules) which had been transformed with human POMC gene. From the electron microscopic localization patterns, L42 cells were considered to serve as a model of constitutive pathway without processing of POMC, and A53 cells were considered to serve as a model of transgranular (regulated) pathway with processing of POMC. Immunoblotting supported these interpretations.

本文言語English
ページ(範囲)503-507
ページ数5
ジャーナルPeptides
12
3
DOI
出版ステータスPublished - 01-01-1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 生理学
  • 内分泌学
  • 細胞および分子神経科学

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「Secretory pathways and processing of human proopiomelanocortin (POMC) using transformed mouse cultured fibroblasts (L cells) and AtT20 cells by human POMC gene-Preembedding immunoelectron microscopic studies」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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