Selective inhibition of DNA polymerase ε by phosphatidylinositol

Mami Shoji-kawaguchi, Shunji Izuta, Keiko Tamiya-koizumi, Motoshi Suzuki, Shonen Yoshida

研究成果: Article査読

32 被引用数 (Scopus)

抄録

We studied the effects of various phoapholipids on the DNA synthesizing reactions by calf thymus DNA polymerases α, δ, and ε. Of these three enzymes, DNA polymerase ε was most sensitive to acidic phospholipids, i.e., phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP), phosphatidylserine (PS), phosphatidic acid (PA), and cardiolipin (CAR). Of these acidic phospholipids, PI (from bovine liver) is of special interest because it inhibited DNA polymerase ε much more strongly than DNA polymerase α and δ. The inhibition of DNA polymerase ε by PI was competitive with the DNA template-primer and was noncompetitive with dTTP substrate. The K1 value was estimated to be 16 μM. These results indicate that PI from bovine liver can be used as a specific inhibitor for DNA polymerase ε to analyze its role in DNA replication. Interestingly, the PI isolated from soybean, which has a different fatty acid composition, inhibited not only DNA polymerase ε but also DNA polymerase α.

本文言語English
ページ(範囲)1095-1099
ページ数5
ジャーナルJournal of Biochemistry
117
5
DOI
出版ステータスPublished - 05-1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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