TY - GEN
T1 - Sensitivity-improved immunoassay for SARS-CoV-2 spike protein in saliva without pretreatment by using immuno-wall microfluidic device
AU - Zhou, Xiang
AU - Kasama, Toshihiro
AU - Miyake, Ryo
N1 - Publisher Copyright:
© 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2022
Y1 - 2022
N2 - In previous publication, we reported a sandwich-shape immunoassay for SARS-CoV-2 spike protein in saliva without pretreatment by using an immuno-wall microfluidic device (IMD). The assay time was 30 min, and the limit of detection (LOD) was 10 - 50 ng/mL. In this study, we developed a method to improve the sensitivity of IMD by using a designed automatic microfluidics delivery system for analyte enrichment. Through this method, the assay time was reduced to about 20 min and the LOD was improved to less than 1 ng/mL. The improved IMD can be applied to the rapid and ultrasensitive test for COVID-19 in the future.
AB - In previous publication, we reported a sandwich-shape immunoassay for SARS-CoV-2 spike protein in saliva without pretreatment by using an immuno-wall microfluidic device (IMD). The assay time was 30 min, and the limit of detection (LOD) was 10 - 50 ng/mL. In this study, we developed a method to improve the sensitivity of IMD by using a designed automatic microfluidics delivery system for analyte enrichment. Through this method, the assay time was reduced to about 20 min and the LOD was improved to less than 1 ng/mL. The improved IMD can be applied to the rapid and ultrasensitive test for COVID-19 in the future.
KW - COVID-19
KW - high-sensitive immunoassay
KW - SARS-CoV-2 spike protein
UR - https://www.scopus.com/pages/publications/85175698595
UR - https://www.scopus.com/pages/publications/85175698595#tab=citedBy
M3 - Conference contribution
AN - SCOPUS:85175698595
T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1197
EP - 1198
BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Y2 - 23 October 2022 through 27 October 2022
ER -