Serial analysis of gene expression in a microglial cell line

Haruhisa Inoue, Makoto Sawada, Akihide Ryo, Hiroshi Tanahashi, Toru Wakatsuki, Akiyuki Hada, Nobuo Kondoh, Keiko Nakagaki, Keikichi Takahashi, Akio Suzumura, Mikio Yamamoto, Takeshi Tabira

研究成果: Article査読

44 被引用数 (Scopus)

抄録

We used the serial analysis of gene expression (SAGE) method to systematically analyze transcripts present in a microglial cell line. Over 10,000 SAGE tags were sequenced, and shown to represent 6,013 unique transcripts. Among the diverse transcripts that had not been previously detected in microglia were those for cytokines such as endothelial monocyte-activating polypeptide I (EMAP I), and for cell surface antigens, including adhesion molecules such as CD9, CD53, CD107a, CD147, CD162 and mast cell high affinity IgE receptor. In addition, we detected transcripts that were characteristic of hematopoietic cells or mesodermal structures, such as E3 protein, A1, EN-7, B94, and ufo. Furthermore, the profile contained a transcript, Hn1, that is important in hematopoietic cells and neurological development (Tang et al. Mamm Genome 8:695-696, 1997), suggesting the probable neural differentiation of microglia from the hematopoietic system in development. Messenger RNA expression of these genes was confirmed by RT-PCR in primary cultures of microglia. Significantly, this is the first systematic profiling of the genes expressed in a microglial cell line. The identification and further characterization of the genes described here should provide potential new targets for the study of microglial biology.

本文言語English
ページ(範囲)265-271
ページ数7
ジャーナルGLIA
28
3
DOI
出版ステータスPublished - 12-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経学
  • 細胞および分子神経科学

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