SHATI/NAT8L regulates neurite outgrowth via microtubule stabilization

Kazuya Toriumi, Miki Ikami, Mizuki Kondo, Akihiro Mouri, Takenao Koseki, Daisuke Ibi, Yoko Furukawa-Hibi, Taku Nagai, Takayoshi Mamiya, Atsumi Nitta, Kiyofumi Yamada, Toshitaka Nabeshima

研究成果: Article

8 引用 (Scopus)

抜粋

We previously identified a new molecule, "SHATI/NAT8L," which has an inhibitory effect on methamphetamine (METH)-induced hyperlocomotion, sensitization, and conditioned place preference. Nevertheless, the extent of SHATI localization and its functions are only partially understood. In this study, we used the FLAG-tag method to investigate SHATI localization. We found that SHATI was localized to microtubules when expressed in COS7 cells and cortical primary neurons. This distribution of SHATI was less apparent after cells were treated with colchicine, a tubulin polymerization inhibitor that disrupts the microtubule structure. This finding suggests that SHATI is associated with microtubule structure. Interestingly, overexpression of SHATI in COS7 cells could attenuate the colchicine-induced decrease in acetylated microtubules, indicating that SHATI plays a role in stabilizing microtubules. Furthermore, we showed that Shati deletion impaired neurite elongation. In cortical primary neurons, neurite length and complexity in Shati-knockout (KO) mice were significantly decreased. In pyramidal neurons in the prefrontal cortex, dendrite length and complexity were also significantly decreased in Shati-KO mice compared with wild-type mice. These results suggest a novel function for SHATI, which may be a new member of the microtubule-associated protein family.

元の言語English
ページ(範囲)1525-1532
ページ数8
ジャーナルJournal of Neuroscience Research
91
発行部数12
DOI
出版物ステータスPublished - 01-12-2013
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

これを引用

Toriumi, K., Ikami, M., Kondo, M., Mouri, A., Koseki, T., Ibi, D., Furukawa-Hibi, Y., Nagai, T., Mamiya, T., Nitta, A., Yamada, K., & Nabeshima, T. (2013). SHATI/NAT8L regulates neurite outgrowth via microtubule stabilization. Journal of Neuroscience Research, 91(12), 1525-1532. https://doi.org/10.1002/jnr.23273