Silencing of FUS in the common marmoset (Callithrix jacchus) brain via stereotaxic injection of an adeno-associated virus encoding shRNA

Kuniyuki Endo, Shinsuke Ishigaki, Yoshito Masamizu, Yusuke Fujioka, Akiya Watakabe, Tetsuo Yamamori, Nobuhiko Hatanaka, Atsushi Nambu, Haruo Okado, Masahisa Katsuno, Hirohisa Watanabe, Masanori Matsuzaki, Gen Sobue

研究成果: Article

3 引用 (Scopus)

抜粋

Fused in sarcoma (FUS) is an RNA binding protein that is involved in frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). To establish the common marmoset (Callithrix jacchus) as a model for FTLD, we generated a stereotaxic injection-based marmoset model of FUS-silencing. We designed shRNAs against the marmoset FUS gene and generated an AAV9 virus encoding the most effective shRNA against FUS (shFUS). The AAV encoding shFUS (AAV-shFUS) was introduced into the frontal cortex of young adult marmosets, whereas AAV encoding a control shRNA was injected into the contralateral side. We obtained approximately 70–80% silencing of FUS following AAV-shFUS injection. Interestingly, FUS-silencing provoked a proliferation of astrocytes and microglias. Since FTLD is characterized by various emotional deficits, it would be helpful to establish a marmoset model of FUS-silencing in various brain tissues for investigating the pathomechanism of higher cognitive and behavioral dysfunction.

元の言語English
ページ(範囲)56-64
ページ数9
ジャーナルNeuroscience Research
130
DOI
出版物ステータスPublished - 05-2018
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

フィンガープリント Silencing of FUS in the common marmoset (Callithrix jacchus) brain via stereotaxic injection of an adeno-associated virus encoding shRNA' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Endo, K., Ishigaki, S., Masamizu, Y., Fujioka, Y., Watakabe, A., Yamamori, T., Hatanaka, N., Nambu, A., Okado, H., Katsuno, M., Watanabe, H., Matsuzaki, M., & Sobue, G. (2018). Silencing of FUS in the common marmoset (Callithrix jacchus) brain via stereotaxic injection of an adeno-associated virus encoding shRNA. Neuroscience Research, 130, 56-64. https://doi.org/10.1016/j.neures.2017.08.006