Simultaneous determination of N2-(3-aminopropyl)biopterin (oncopterin), biopterin and neopterin by high-performance liquid chromatography with fluorescence detection

M. Hibiya, R. Teradaira, T. Sugimoto, K. Fujita, T. Nagatsu

研究成果: Article査読

12 被引用数 (Scopus)


A high-performance liquid chromatographic method is described for the simultaneous determination of N2-(3-aminopropyl)biopterin (oncopterin, a newly found natural pteridine in urine from cancer patients), biopterin, and neopterin in urine. For the detection and quantification of the compounds, fluorometry was used. Using Develosil ODS K-5 and Develosil ODS HG-5 reversed-phase columns and a Nucleosil 100-5SA strong cation-exchange column, oncopterin, biopterin, and neopterin in urine were completely separated and assayed simultaneously by fluorescence detection. Similar values of oncopterin were obtained using each of the three columns, and the Develosil ODS K-5 reversed-phase column gave the most satisfactory separation. The sensitivity was high enough to measure 1 pmol of each pteridine. The HPLC method was highly reproducible. Our preliminary results indicate that oncopterin could be a most sensitive marker for cancer.

ジャーナルJournal of Chromatography B: Biomedical Sciences and Applications
出版ステータスPublished - 06-10-1995

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

フィンガープリント 「Simultaneous determination of N<sup>2</sup>-(3-aminopropyl)biopterin (oncopterin), biopterin and neopterin by high-performance liquid chromatography with fluorescence detection」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。