Single chain variable fragment antibodies against shiga toxins isolated from a human antibody phage display library

Paola Neri, Naoko Shigemori, Susumu Hamada-Tsutsumi, Kentaro Tsukamoto, Hideyuki Arimitsu, Toshiyasu Shimizu, Yasushi Akahori, Yoshikazu Kurosawa, Takao Tsuji

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Shiga toxins (Stxs) are involved in the pathogenesis of hemolytic-uremic syndrome and other severe systemic complications following enterohemorrhagic Escherichia coli infection in humans. Passive immunotherapies using monoclonal antibodies have been shown to be effective for neutralizing the toxic effects of Stxs. However, animal-derived monoclonal antibodies are sometimes immunogenic and their production is both laborious and expensive. We here report the isolation of single-chain variable fragment antibodies against Stxs by screening a phage display library constructed from a naïve human repertoire. An antibody among the selected clones designated B22 bound to the binding subunits of both Stx-1 and Stx-2, and strongly neutralized the cytotoxicity of Stx-1. This is the first example of a monovalent antibody showing Stx-neutralizing activity. The B22 antibody is also completely naturally occurring in human, which reduces the possibility of adverse immunological effects, and can be easily produced using bacterial protein synthesis systems.

本文言語English
ページ(範囲)5340-5346
ページ数7
ジャーナルVaccine
29
33
DOI
出版ステータスPublished - 26-07-2011

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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