Single-tube multiplex polymerase chain reaction for the detection of genes encoding enterobacteriaceae carbapenemase

Masanori Watahiki, Ryuji Kawahara, Masahiro Suzuki, Miyako Aoki, Kaoru Uchida, Yuko Matsumoto, Yuko Kumagai, Makiko Noda, Kanako Masuda, Chiemi Fukuda, Seiya Harada, Keiko Senba, Masato Suzuki, Mari Matsui, Satowa Suzuki, Keigo Shibayama, Hiroto Shinomiya

研究成果: Article査読

2 被引用数 (Scopus)

抄録

A multiplex PCR assay in a single tube was developed for the detection of the carbapenemase genes of Enterobacteriaceae. Primers were designed to amplify the following six carbapenemase genes: bla bla bla bla bla and bla Of 70 blaIMP variants, 67 subtypes were simulated toKPC,be PCR-positiveIMP,NDM,basedVIM,on in silicoOXA-48-like,simulation andGES.the primer-design strategy. After determining the optimal PCR conditions and performing in vitro assays, the performance of the PCR assay was evaluated using 51 and 91 clinical isolates with and without carbapenemase genes, respectively. In conclusion, the combination of multiplex PCR primers and QIAGEN Multiplex PCR Plus Kit was used to determine the best performance for the rapid and efficient screening of carbapenemase genes in Enterobacteriaceae. The assay had an overall sensitivity and specificity of 100%. This PCR assay compensates for the limitations of phenotypic testing, such as antimicrobial susceptibility testing and the modified carbapenem inactivation method, in clinical and public health settings.

本文言語English
ページ(範囲)166-172
ページ数7
ジャーナルJapanese journal of infectious diseases
73
2
DOI
出版ステータスPublished - 2020

All Science Journal Classification (ASJC) codes

  • 微生物学(医療)
  • 感染症

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