Soluble CXCL16 in preoperative serum is a novel prognostic marker and predicts recurrence of liver metastases in colorectal cancer patients

Kohei Matsushita, Yuji Toiyama, Koji Tanaka, Susumu Saigusa, Junichiro Hiro, Keiichi Uchida, Yasuhiro Inoue, Masato Kusunoki

研究成果: ジャーナルへの寄稿学術論文査読

49 被引用数 (Scopus)

抄録

Purpose: This study was designed to identify novel and reliable serum prognostic markers in patients with colorectal cancer (CRC). Methods: Based on cytokine array analysis, we identified soluble CXCL16 as a novel prognostic serum marker. Serum levels of CXCL16 were assessed in 314 CRC patients and 20 normal volunteers by enzyme-linked immunosorbent assay, and their relationships with clinicopathologic findings, including survival, were investigated. Proliferation, invasion, and wound healing assays were used to investigate the biological role of soluble CXCL16 in CRC cells, by exposure of HT-29 cells to recombinant CXCL16. Results: The median serum CXCL16 concentration in CRC patients was significantly higher than that in normal volunteers. In addition, serum CXCL16 levels increased significantly in accordance with the progression of UICC stage classification. Elevated serum CXCL16 level was significantly associated with poor survival and was an independent prognostic marker in CRC patients. Furthermore, in stage I-III CRC patients who underwent curative intent surgery, elevated serum CXCL16 levels were significantly associated with metachronous liver recurrence and poor survival. Recombinant soluble CXCL16 promoted the epithelial-mesenchymal transition (EMT) phenotype characterized by impaired E-cadherin production and induction of vimentin in vitro. In addition, recombinant soluble CXCL16 promoted cell growth, migration, and invasion in a CRC cell line. Conclusions: In this study, we identified CXCL16 as a novel prognostic marker. Preoperative high serum levels of CXCL16 were associated with metachronous liver recurrence and poor prognosis in CRC patients. Soluble CXCL16 may play an important role in liver metastases through the induction of EMT.

本文言語英語
ページ(範囲)S518-S527
ジャーナルAnnals of Surgical Oncology
19
SUPPL. 3
DOI
出版ステータス出版済み - 07-2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 外科
  • 腫瘍学

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