Spatiotemporal expression of BDNF in the hippocampus induced by the continuous intracerebroventricular infusion of β-amyloid in rats

Ya Ping Tang, Kiyofumi Yamada, Yasuhiko Kanou, Takashi Miyazaki, Xiao Li Xiong, Fukushi Kambe, Yoshiharu Murata, Hisao Seo, Toshitaka Nabeshima

研究成果: Article査読

34 被引用数 (Scopus)

抄録

The β-amyloid protein (Aβ) is the major component of senile plaques found in the brain in Alzheimer's disease (AD). Its neurotoxic properties in vivo, however, are not well defined. Since the expression of neurotrophin genes is considered an important component of the intrinsic neuroprotective response to insults, we analyzed the gene expression of neurotrophins in the brains of rats which received a continuous infusion of Aβ-(1-42) into the cerebroventricle. Northern blot analysis revealed a significant increase in brain-derived neurotrophic factor (BDNF) expression in the hippocampus but no change in the cerebral cortices. The alteration peaked on days 3-7 and returned to the basal level on day 14 after the start of Aβ-(1-42) infusion. No significant changes in nerve growth factor or neurotrophin-3 mRNA expression were observed. The infusion of Aβ-(1-40) and (25-35) also triggered the expression of BDNF mRNA, whereas neither Aβ-(40-1) nor (1-16) had any effect. In situ hybridization histochemistry revealed that the expression mainly occurred in the hilus and granular layer of the dentate gyrus and to a lesser extent in the pyramidal neurons of the CA1 region. These results demonstrate that the continuous intracerebroventricular infusion of Aβ induces selective and spatiotemporal expression of BDNF mRNA in the hippocampus. (C) 2000 Elsevier Science B.V.

本文言語English
ページ(範囲)188-197
ページ数10
ジャーナルMolecular Brain Research
80
2
DOI
出版ステータスPublished - 15-09-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞および分子神経科学

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