SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns

Kazuhiro Fukumura, Rei Yoshimoto, Luca Sperotto, Hyun Seo Kang, Tetsuro Hirose, Kunio Inoue, Michael Sattler, Akila Mayeda

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Human pre-mRNA introns vary in size from under fifty to over a million nucleotides. We searched for essential factors involved in the splicing of human short introns by screening siRNAs against 154 human nuclear proteins. The splicing activity was assayed with a model HNRNPH1 pre-mRNA containing short 56-nucleotide intron. We identify a known alternative splicing regulator SPF45 (RBM17) as a constitutive splicing factor that is required to splice out this 56-nt intron. Whole-transcriptome sequencing of SPF45-deficient cells reveals that SPF45 is essential in the efficient splicing of many short introns. To initiate the spliceosome assembly on a short intron with the truncated poly-pyrimidine tract, the U2AF-homology motif (UHM) of SPF45 competes out that of U2AF65 (U2AF2) for binding to the UHM-ligand motif (ULM) of the U2 snRNP protein SF3b155 (SF3B1). We propose that splicing in a distinct subset of human short introns depends on SPF45 but not U2AF heterodimer.

本文言語English
論文番号4910
ジャーナルNature communications
12
1
DOI
出版ステータスPublished - 01-12-2021

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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