Stimulation of TIMP-1 production by oncostatin M in human articular cartilage

Osamu Nemoto, Harumoto Yamada, Masahiro Mukaida, Masayuki Shimmei

研究成果: Article

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Objective. To investigate the effects of the interleukin-6 (IL-6) family cytokines, such as IL-6, IL-11, leukemia inhibitory factor (LIF), and oncostatin M (OSM), on the production of tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) in human articular cartilage. Methods. Effects of IL-6 family cytokines and growth factors on TIMP-1 production were studied in human articular chondrocytes, grown as monolayer and cartilage explant culture. TIMP-1 protein levels in the cultured medium were measured by sandwich enzyme immunoassay. TIMP-1 messenger RNA levels in the cultured chondrocytes were analyzed by Northern blotting. Western blot analysis was performed to evaluate the release of matrix metalloproteinases (MMPs) in the cultured medium. Cell proliferation of chondrocytes was determined by 3H- thymidine uptake. Results. IL-6 family cytokines induced TIMP-1 expression in monolayer and explant culture, and the production of TIMP-1 was most stimulated by OSM. In contrast, OSM did not modulate the release of MMPs and cell proliferation. Conclusion. These results suggest that OSM may be characterized as one of the chondroprotective mediators in cartilage destruction.

元の言語English
ページ(範囲)560-566
ページ数7
ジャーナルArthritis and Rheumatism
39
発行部数4
DOI
出版物ステータスPublished - 01-04-1996
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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