Structure and function of the pancreas in the polycystic kidney rat

Lanjuan Yi, Satoru Naruse, Sonoko Furuya, Akiko Yamamoto, Miyuki Nakakuki, Shizuko Nagao, Daisuke Yoshihara, Shigeru B.H. Ko, Muxin Wei, Takaharu Kondo, Hiroshi Ishiguro

研究成果: ジャーナルへの寄稿学術論文査読

2 被引用数 (Scopus)

抄録

OBJECTIVES: Mutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats. METHODS: Pancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry. RESULTS: Histologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P < 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H2O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats. CONCLUSIONS: These findings suggest that fibrocystin/primary cilia-dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.

本文言語英語
ページ(範囲)1292-1298
ページ数7
ジャーナルPancreas
41
8
DOI
出版ステータス出版済み - 11-2012

All Science Journal Classification (ASJC) codes

  • 内科学
  • 内分泌学、糖尿病および代謝内科学
  • 肝臓学
  • 内分泌学

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